SIMULTANEOUS DETERMINATION OF A NOVEL ANTICANCER DRUG, TAS-103, AND ITS N-DEMETHYLATED METABOLITE IN MONKEY PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING SOLID-PHASE EXTRACTION
R. Azuma et A. Urakawa, SIMULTANEOUS DETERMINATION OF A NOVEL ANTICANCER DRUG, TAS-103, AND ITS N-DEMETHYLATED METABOLITE IN MONKEY PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING SOLID-PHASE EXTRACTION, Journal of chromatography B. Biomedical sciences and applications, 691(1), 1997, pp. 179-185
Citations number
6
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
A simple and rapid method for the analysis of a novel anticancer drug,
TAS-103, and its metabolite demethyl-TAS-103 in monkey plasma has bee
n developed. This method is based on high-performance liquid chromatog
raphy with visible detection at 460 nm after solid-phase extraction wi
th a Sep-Pak Vac PS-2 cartridge. The extraction recoveries of each com
pound, including the internal standard TAS-1-018, were from 88 to 102%
. The quantitation limit of each compound was 5.0 ng/ml in 0.5 ml of p
lasma. The coefficients of variation for each compound ranged from 0.9
to 4.9%, and relative errors for each compound ranged from -3.8 to 4.
6%. Both compounds in monkey plasma were stable at -80 degrees C for 3
9 days and the extracts were stable at ambient temperature for 24 h. T
his method has been demonstrated to be useful for the pharmacokinetic
study of TAS-103 in monkey plasma after intravenous administration.