Mt. Chaudhary et al., ION ACCUMULATION IN LUCERNE PLANTS REGENERATED FROM SALT ADAPTED SUSPENSION-CULTURES COMPARED WITH RECULTURED CELLS FROM THESE PLANTS, Plant cell reports, 17(2), 1997, pp. 145-149
Lucerne (Medicago media cv. Rambler) plants, regenerated from cell sus
pension cultures adapted to 200 mM NaCl, and cell cultures derived fro
m the leaflets of regenerated plants showed enhanced Na+ exclusion com
pared with the unselected plants and cell cultures. There was a decrea
se in the K+ concentration in response to NaCl treatment but the selec
ted plants and cell cultures maintained a significantly higher K+ conc
entration and showed much lower Na+/K+ ratios than unselected plants a
nd cell cultures. The proline concentration increased in the shoots an
d cell cultures in response to NaCl stress but the selected plants and
cell cultures showed a significantly higher proline concentration tha
n unselected plants and cell cultures.