CDNA CLONING OF THE TRANSLOCON ASSOCIATED PROTEIN BETA-SUBUNIT IN THECHICK CEREBELLUM

The 'translocon associated protein' is a tetrameric complex residing i n the translocation sites, in which nascent polypeptides pass through the endoplasmic reticulum membrane. The beta subunit of this complex i s a single spanning membrane protein, as deduced from cDNAs deriving f rom canine or human epithelial tissues. We have isolated and analysed a cDNA clone of the beta subunit from chick nervous tissue, namely cer ebellum. Its deduced protein sequence is 91% homologous to both the ca nine and the human protein sequences, showing that the molecule is hig hly conserved. (C) 1997 Elsevier Science B.V.