T. Sato et al., CHARACTERIZATION, CELL UPTAKE, AND SUBCELLULAR-DISTRIBUTION OF DNA COMPLEXES WITH LIPOGLUTAMIDES HAVING TETRAETHYLENE GLYCOL TAILS, Journal of biomaterials science. Polymer ed., 9(1), 1997, pp. 31-42
Lipoglutamides with tetraethylene glycol tails were synthesized. Physi
cochemical features of the DNA/lipoglutamide complexes were investigat
ed by light scattering method, phase transition, and CD-spectrum. Aggr
egation of the DNA/lipoglutamide complex was significantly depressed c
ompared with DNA complexes without ethylene glycol tails, and the solu
tion showed no turbidity. The DNA/lipoglutamide complex showed a high
resistance to nuclease, and an efficient internalization into tumor ce
lls, compared with those of DNA alone. Furthermore, the DNA complex wa
s found by confocal laser scanning fluorescence microscopy to distribu
te in the cytoplasmic region.