In a phylogenetic analysis of the tribe Poeciliini, three of four subg
enera of Poecilia (i.e., Limia, Pamphorichthys, and Poecilia) are reco
gnized as genera based on the derived features of the gonopodium and i
ts suspensorium. Based on the Hollister-foramen and a keel on the subd
istal ventral side of ray 5 of the gonopodium, Limia, Pamphorichthys,
and Poecilia comprise a monophyletic group. Xiphophorus is the sister
taxon of the clade (Pamphorichthys, Poecilia, Limia). Neither Alfaro n
or Priapella is known to share derived characters with the clade Xipho
phorus (Limia, Pamphorichthys, and Poecilia). Falsification of the hyp
othesis of Priapella and Xiphophorus as sister taxa is discussed.