GROWTH-INHIBITION OF HELICOBACTER-PYLORI BY MONOCLONAL-ANTIBODY TO HEAT-SHOCK-PROTEIN-60

The H20mAb recognizing the 60-kilodalton protein, which existed in the outer membrane and was induced by heat shock at 42 C, was established , The molecule recognized with the mAb was a heat-shock protein 60 (HS P60) of Helicobacter pylori, To understand the role of HSP60 on the ce ll surface of H. pylori, whether or not H20mAb affects the growth of H . pylori was investigated, When bacteria were cultured with H20mAb, gr owth was markedly inhibited after 24 hr, although an initial 5 hr-incu bation with the mAb induced no significant inhibition of H. pylori gro wth, The 24- and 48 hr growth of the bacteria after washing to remove the mAb at 5 hr was also inhibited though the inhibitory effect was no t strong, In electron microscopical analysis, the spots with high elec tron density in the cytoplasm of the bacteria treated with H20mAb were increased, depending on the length of incubation time from 5 to 24 hr . After 24 hr treatment with H20mAb, bacterial destruction was also ob served, indicating bactericidal activity by H20mAb, These results sugg est that the HSP60 on the cell surface of H. pylori might have an esse ntial role in the growth of the bacteria.