CLONING AND STRUCTURAL-ANALYSIS OF THE MURINE GCN5L1 GENE

We recently cloned the murine 11-cis retinol dehydrogenase gene. A sec ond gene, the murine GCN5L1 gene, was found to be situated upstream of the murine 11-cis retinol dehydrogenase gene. We have isolated and se quenced the complete coding sequence of the murine GCN5L1 gene. The di stance between the 3'-end of the murine GCN5L1 gene and the 5'-end of the 11-cis retinol dehydrogenase gene is only 776 nt. The murine GCN5L 1 gene consists of four exons encompassing approximately 3.5 kb of gen omic DNA. Intron/exon splice sites conform to the GT/AG rule. The open reading frame consists of 375 nucleotides encoding a 14 kDa protein. The murine GCN5L1, like the human GCN5L1 protein, displays weak homolo gy (27%) to yeast GCN5. The distance between the murine, human and bov ine GCN5L1 and 11-cis retinol dehydrogenase genes appeared to be conse rved. (C) 1997 Elsevier Science B.V.