CONSTRUCTION OF A GREEN-FLUORESCENT PROTEIN-BASED, INSERTION-INACTIVATION SHUTTLE VECTOR FOR LACTIC-ACID BACTERIA AND ESCHERICHIA-COLI

Authors
SOLAIMAN DKY SOMKUTI GA
Citation
Dky. Solaiman et Ga. Somkuti, CONSTRUCTION OF A GREEN-FLUORESCENT PROTEIN-BASED, INSERTION-INACTIVATION SHUTTLE VECTOR FOR LACTIC-ACID BACTERIA AND ESCHERICHIA-COLI, Biotechnology letters, 19(12), 1997, pp. 1175-1179
Citations number
15
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
19
Issue
12
Year of publication
1997
Pages
1175 - 1179
Database
ISI
SICI code
0141-5492(1997)19:12<1175:COAGPI>2.0.ZU;2-A
Abstract
A shuttle vector, p5aGFP2201a, for lactic acid bacteria and E. coli wa s constructed by using the gene of a jellyfish green fluorescent prote in (gfp) as a selection marker. The plasmid was shown to function as a shuttle vector by its ability to carry and express a staphylococcal c hloramphenicol acetyltransferase (cat) gene into targeted hosts.