CLONING AND SEQUENCING OF AN ASPERGILLUS-NIGER GENE CODING FOR BETA-FRUCTUFURANOSIDASE

Authors
SOMIARI RI BRZESKI H TATE R BIELECK S POLAK J
Citation
Ri. Somiari et al., CLONING AND SEQUENCING OF AN ASPERGILLUS-NIGER GENE CODING FOR BETA-FRUCTUFURANOSIDASE, Biotechnology letters, 19(12), 1997, pp. 1243-1247
Citations number
19
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
19
Issue
12
Year of publication
1997
Pages
1243 - 1247
Database
ISI
SICI code
0141-5492(1997)19:12<1243:CASOAA>2.0.ZU;2-M
Abstract
A 1.7 kb DNA fragment encoding beta-D-fructofuranosidase in a strain o f Aspergillus niger was amplified by PCR, inserted into a 4.4 kb cloni ng vector and sequenced. Comparison of this sequence with suc1 (GenBan k) showed that this invertase gene had suffered a single base deletion followed by a single base insertion 49bp further down-stream with the result that 16 amino acids are coded by a different reading frame.