Ra. Dezeeuw, DRUG SCREENING IN BIOLOGICAL-FLUIDS - THE NEED FOR A SYSTEMATIC-APPROACH, Journal of chromatography B. Biomedical sciences and applications, 689(1), 1997, pp. 71-79
Citations number
27
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
In this paper the key steps towards drug screening in biological fluid
s are considered: (i) sample work up-isolation-concentration: (ii) dif
ferentiation-detection; (iii) identification. For (i) solid-phase extr
action has very good potential; for (ii) thin-layer chromatography, ga
s chromatography and high-performance liquid chromatography, in combin
ation with their respective detection modes, each have their particula
r advantages and disadvantages; for (iii) extensive computerized data
bases are essential. It is emphasized that each step must be approache
d and carried out in a systematic way, not only to make sure that all
substances of interest can be detected, but also for their correct ide
ntification. To this end all analytical procedures and techniques used
require extensive validation of their detection and identification pr
operties.