GAS-CHROMATOGRAPHY MASS-SPECTROMETRY (GC-MS) AND LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY (LC-MS) IN TOXICOLOGICAL ANALYSIS - STUDIES ON THEDETECTION OF CLOBENZOREX AND ITS METABOLITES WITHIN A SYSTEMATIC TOXICOLOGICAL ANALYSIS PROCEDURE BY GC-MS AND BY IMMUNOASSAY AND STUDIES ON THE DETECTION OF ALPHA-AMANITIN AND BETA-AMANITIN IN URINE BY ATMOSPHERIC-PRESSURE IONIZATION ELECTROSPRAY LC-MS

GC-MS is the method of choice for toxicological analysis of toxicants volatile in GC while non-volatile and/or thermally labile toxicants ne ed LC-MS for their determination. Studies are presented on the toxicol ogical detection of the amphetamine-like anorectic clobenzorex in urin e by GC-MS after acid hydrolysis, extraction and acetylation and by fl uorescence polarization immunoassay (FPIA, TDx (meth)amphetamine II). After ingestion of 60 mg of clobenzorex, the parent compound and/or it s metabolites could be detected by GC-MS for up to 84 h or by FPIA for up to 60 h. Since clobenzorex shows no cross-reactivity with the used immunoassay, the N-dealkylated metabolite amphetamine is responsible for the positive TDx results. The intake of clobenzorex instead of amp hetamine can be differentiated by GC-MS detection of hydroxyclobenzore x which is detectable for at least as long as amphetamine. In addition , the described GC-MS procedure allows the simultaneous detection of m ost of the toxicologically relevant drugs. Furthermore, studies are de scribed on the atmospheric pressure ionization electrospray LC-MS dete ction of alpha- and beta-amanitin, toxic peptides of amanita mushrooms , in urine after solid-phase extraction on RP-18 columns. Using the si ngle ion monitoring mode with the ions m/z 919 and 920 the amanitins c ould be detected down to 10 ng/ml of urine which allows us to diagnose intoxications with amanita mushrooms.