H. Lingeman et Sjf. Hoekstraoussoren, PARTICLE-LOADED MEMBRANES FOR SAMPLE CONCENTRATION AND OR CLEANUP IN BIOANALYSIS/, Journal of chromatography B. Biomedical sciences and applications, 689(1), 1997, pp. 221-237
Citations number
83
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Solid-phase extraction nowadays is a major sample preparation tool. Th
e latest development in this area is the introduction of particle-load
ed membranes (membrane-extraction disks). The potential of these extra
ction membranes in bioanalysis is discussed with respect to recoveries
, reproducibility, sensitivity and speed. A comparison is made between
liquid-liquid extraction and solid-phase extraction using traditional
sorbents and extraction disks, and off-line and on-line techniques. P
article-loaded membranes are available in disks with diameters of 4-90
mm. The 25-90 mm disks are mainly used for off-line extractions of ma
inly environmental samples, while the 4 mm disks are available in the
so-called drug tubes that can be used in the same way as conventional
extraction cartridges for the extraction of drugs from biological flui
ds. The main advantage of using drug tubes is the smaller desorption v
olume and, therefore, the increased sensitivity. Cutting smaller disks
, from the commercially available disks, allows the use of on-line ext
ractions in column-switching systems. The main conclusion is that in m
any cases particle-loaded membranes are more efficient than packed sol
id-phase extraction cartridges.