DETERMINATION OF MELANOTAN-II IN RABBIT URINE USING SOLID-PHASE EXTRACTION SAMPLE PREPARATION FOLLOWED BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Authors
WALTERS AE MYRDAL PB PINSUWAN S MANKA AM YALKOWSKY SH
Citation
Ae. Walters et al., DETERMINATION OF MELANOTAN-II IN RABBIT URINE USING SOLID-PHASE EXTRACTION SAMPLE PREPARATION FOLLOWED BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography B. Biomedical sciences and applications, 690(1-2), 1997, pp. 99-103
Citations number
8
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications → ACNP
ISSN journal
13872273
Volume
690
Issue
1-2
Year of publication
1997
Pages
99 - 103
Database
ISI
SICI code
0378-4347(1997)690:1-2<99:DOMIRU>2.0.ZU;2-O
Abstract
A solid-phase extraction (SPE) method has been developed for the isola tion of melanotan II from rabbit urine. The proposed extraction method makes it possible to selectively isolate melanotan II without signifi cant loss of the peptide. Standard curves obtained from high-performan ce liquid chromatographic (HPLC) analysis of spiked urine extracts are linear from 0.1 to 4.0 mu g/ml. The analytical method is shown to be highly reproducible, giving a relative standard deviation of less than 5% for both between-day and same-day analyses. The accuracy of the me thod obtained from standard plots ranges from -3.3 to 3.1%.