CHROMATOGRAPHIC ANALYSIS OF THE ENANTIOMERS OF IFOSFAMIDE AND SOME OFITS METABOLITES IN PLASMA AND URINE

The enantiomers of the cytostatic drug ifosfamide and the two metaboli tes 2- and 3-dechloroethylifosfamide were isolated from plasma and uri ne by liquid-liquid extraction with ethyl acetate, ri:solved on a Chir asil-L-val gas chromatographic column and detected by a nitrogen-phosp horus-selective flame ionisation detector. Resolution of the racemic c ompounds for identification purposes was also accomplished with high-p erformance liquid chromatography on a chiral column. The validated gas chromatographic method was suitable to determine the total concentrat ions and the enantiomeric composition of ifosfamide and its dechloroet hylated metabolites in plasma and urine: samples from treated patients . Some metabolic preferences in the metabolism of ifosfamide were foun d.