ANALYSIS OF DIPALMITOYL PHOSPHATIDYLCHOLINE IN AMNIOTIC-FLUID BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Authors
ALVAREZ JG SLOMOVIC B LUDMIR J
Citation
Jg. Alvarez et al., ANALYSIS OF DIPALMITOYL PHOSPHATIDYLCHOLINE IN AMNIOTIC-FLUID BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography B. Biomedical sciences and applications, 690(1-2), 1997, pp. 338-342
Citations number
19
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications → ACNP
ISSN journal
13872273
Volume
690
Issue
1-2
Year of publication
1997
Pages
338 - 342
Database
ISI
SICI code
0378-4347(1997)690:1-2<338:AODPIA>2.0.ZU;2-D
Abstract
A novel method for the determination of dipalmitoyl phosphatidylcholin e (DPPC) in amniotic fluid by high-performance liquid chromatography ( HPLC) is described. Aliquots of 50 mu l of amniotic fluid were hydroly zed with phospholipase C from Bacillus cereus and the resulting dipalm itoylglycerol analyzed by HPLC. Run-to-run and day-to-day precision fo r DPPC analysis were 4.2 and 6.1%, respectively, and analysis time was 10 min. Recoveries for DPPC ranged between 92 and 98%. In summarizing , this method provides a high precision and fast turnaround time means for the analysis of DPPC in amniotic fluid.