ASSAY OF SOLUBLE GUANYLATE-CYCLASE ACTIVITY BY ISOCRATIC HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Authors
PIETTA PG MAURI PL GARDANA C BENAZZI L
Citation
Pg. Pietta et al., ASSAY OF SOLUBLE GUANYLATE-CYCLASE ACTIVITY BY ISOCRATIC HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography B. Biomedical sciences and applications, 690(1-2), 1997, pp. 343-347
Citations number
13
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications → ACNP
ISSN journal
13872273
Volume
690
Issue
1-2
Year of publication
1997
Pages
343 - 347
Database
ISI
SICI code
0378-4347(1997)690:1-2<343:AOSGAB>2.0.ZU;2-W
Abstract
A HPLC method alternative to labelled or unlabelled procedures was dev eloped for the assay of guanylate cyclase (GC) activity. The substrate (GTP) and the product (cGMP) of the enzymatic re;action were separate d in the isocratic mode on a mu Bondapak C-18 column. The activity of GC was linearly dependent on the amount of cGMP produced in the presen ce of sodium nitroprusside. This approach was applied to follow the pu rification of GC from bovine lung and to evaluate its stability in dif ferent storage conditions.