AMPEROMETRIC DETERMINATION OF ENZYMATIC-ACTIVITY BY MULTIENZYME BIOSENSORS

A Clark type, amperometric multienzyme electrode employing glucose oxi dase and mutarotase, was used to determine the enzymatic activity of a model enzyme, invertase, in soluble and immobilized forms. A calibrat ion curve with linear range up to 70 IU of invertase activity correspo nding to the 6.0 nA/s of electrode response was sketched. It is shown that, multienzyme electrodes can be utilized as a very rapid, reliable and sensitive tool for the determination of enzymatic activity either in a free or immobilized form. (C) 1997 Elsevier Science Limited. All rights reserved.