LOW-AFFINITY FLUORESCENT INDICATOR FOR INTRACELLULAR CALCIUM-IONS

Authors
TAKESAKO K SASAMOTO K OHKURA Y HIROSE K IINO M
Citation
K. Takesako et al., LOW-AFFINITY FLUORESCENT INDICATOR FOR INTRACELLULAR CALCIUM-IONS, Analytical communications, 34(12), 1997, pp. 391-392
Citations number
8
Journal title
Analytical communications → ACNP
ISSN journal
13597337
Volume
34
Issue
12
Year of publication
1997
Pages
391 - 392
Database
ISI
SICI code
1359-7337(1997)34:12<391:LFIFIC>2.0.ZU;2-B
Abstract
A Ca2+ indicator, minophenoxy)ethoxy]-coumarin-N,N,N',N'-tetraacetic a cid was synthesized, The indicator has a large Kd value of 0.55 mM and shows an excitation spectral shift from 462 to 401 nm upon Ca2+ bindi ng, The indicator, therefore, may be useful for the measurement of Ca2 + concentrations in the endoplasmic reticulum of living cells by ratio metric fluorescence spectroscopy with visible light excitation.