LIVING BACTERIAL-CELLS MICROEXTRACTION AS A TOOL FOR SPECIATION OF ORGANOSELENO COMPOUNDS

A reliable method combining a time-controlled solid phase microextract ion using living bacterial cells and electrothermal atomic absorption spectrometry is proposed for the speciation of organoseleno compounds. The extraction medium consists of a Pseudomonas putida strain cultiva ted in a culture medium based on glucose contaminated with an organose leno compound. The adsorption process study was derived in order to de velop an analytical procedure for determining several organoseleno com pounds (selenomethionine, selenourea, and selenocystamine) in samples containing other selenium species. The best detection limits for the o rganoseleno compounds studied are 0.1-1.5 ng ml(-1). The relative stan dard deviations of the retention and determination procedure are 1.9-5 .6%.