SOME PROPERTIES AND ACTION MODE OF (1-]4)-ALPHA-L-GULURONAN LYASE FROM ENTEROBACTER-CLOACAE M-1

An intracellular alginate lyase was purified from Enterobacter cloacae M-l by successive fractionation on Q Sepharose FF, SP Sepharose FF, a nd Sephacryl S-200 HR. The purified enzyme gave a single band on SDS-P AGE and isoelectric focusing. The enzyme easily degraded polyguluronat e and produced unsaturated oligoguluronic acids with a wide range of d p. The major end product of the enzyme reaction on polyguluronate was unsaturated triuronic acid. The pattern of oligoguluronic acids (dp 2- 9) generated with the enzyme was investigated by fluorophore-assisted carbohydrate electrophoresis. The enzyme was not capable of degrading acids having a dp < 4. The degradation rate of heptaguluronic acid by this enzyme remarkably increased, compared with that of hexaguluronic acid, and heptaguluronic acid had a single preferential point of cleav age by this enzyme. On the basis of the cleavage pattern of oligogulur onic acids, the number of subsites was estimated to be seven for this enzyme. The catalytic site of the enzyme is located between the second and the third subsites from the non-reducing end. (C) 1997 Elsevier S cience Ltd. All rights reserved.