REVERSE-TRANSCRIPTASE POLYMERASE CHAIN-REACTION FOR AMANTADINE SUSCEPTIBILITY TESTING OF INFLUENZA-VIRUSES

Authors
SUEN PC TSAO KC WU TL NING HC LIU TZ SHIH SR
Citation
Pc. Suen et al., REVERSE-TRANSCRIPTASE POLYMERASE CHAIN-REACTION FOR AMANTADINE SUSCEPTIBILITY TESTING OF INFLUENZA-VIRUSES, Biotechnology techniques, 11(12), 1997, pp. 873-878
Citations number
13
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
11
Issue
12
Year of publication
1997
Pages
873 - 878
Database
ISI
SICI code
0951-208X(1997)11:12<873:RPCFAS>2.0.ZU;2-Z
Abstract
The clinical use of amantadine for influenza A viruses infection is in creasing. A novel quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) assay was developed to determine the IC50 (dose of 5 0% inhibitory) of amantadine. The traditional amantadine susceptibilit y testing by plaque inhibition assay took 3 days to observe plaque for mation. In contrast, the proposed assay took 3 hours to detect viral R NA. The RT-PCR products can be directly sequenced for screening and id entifying the resistant strains.