APPLICATION OF FLUOROSOME STOPPED-FLOW SPECTROPHOTOMETRY TO MONITOR THE ENTRY OF MOLECULES INTO LIPID BILAYERS

Authors
MELCHIOR DL MAKRIYANNIS A
Citation
Dl. Melchior et A. Makriyannis, APPLICATION OF FLUOROSOME STOPPED-FLOW SPECTROPHOTOMETRY TO MONITOR THE ENTRY OF MOLECULES INTO LIPID BILAYERS, Biotechnology techniques, 11(12), 1997, pp. 885-888
Citations number
10
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
11
Issue
12
Year of publication
1997
Pages
885 - 888
Database
ISI
SICI code
0951-208X(1997)11:12<885:AOFSST>2.0.ZU;2-O
Abstract
A method employing fluorescent unilammelar vesicles was developed for the in vitro determination of non-protein mediated entry rates of mole cules into biomembranes with half-times of of entry of from millisecon ds to hours. This approach can further determine the equilibrium accum ulation and partitioning ratios of test molecules between membranes an d delivery solutions or carrier molecules.