SEPARATION AND ANALYSIS OF BETA-LACTAM ANTIBIOTICS BY HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS - ENZYMATIC-SYNTHESIS, A CASE-STUDY

Authors
NIERSTRASZ VA SCHROEN CGPH BOSMA R KROON PJ BEEFTINK HH JANSSEN AEM TRAMPER J
Citation
Va. Nierstrasz et al., SEPARATION AND ANALYSIS OF BETA-LACTAM ANTIBIOTICS BY HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS - ENZYMATIC-SYNTHESIS, A CASE-STUDY, Biotechnology techniques, 11(12), 1997, pp. 899-903
Citations number
18
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
11
Issue
12
Year of publication
1997
Pages
899 - 903
Database
ISI
SICI code
0951-208X(1997)11:12<899:SAAOBA>2.0.ZU;2-L
Abstract
High-performance capillary electrophoresis (CE) and HPLC were compared for the analysis of some enzymatically synthesised beta-lactam antibi otics. The main advantage for CE, besides its lower operational costs, is the short analysis time. It was further found that CE could be use d for a variety of antibiotics and their building blocks by small adju stments, affecting the mobility of the solutes, in the analysis method . The compounds studied have a typical detection limit of 10 mu M for both CE and HPLC.