T. Miyazawa et al., MARKED DEPENDENCE ON TEMPERATURE OF ENANTIOSELECTIVITY IN THE ASPERGILLUS-ORYZAE PROTEASE-CATALYZED HYDROLYSIS OF AMINO-ACID ESTERS, Biotechnology techniques, 11(12), 1997, pp. 931-933
In the Aspergillus oryzae protease-catalyzed enantioselective hydrolys
is of amino acid esters, linear relationships were observed between-RT
InE and T, where E=enantiomeric ratio and T=absolute temperature. Acco
rding to this temperature dependence, the E value of 11 at 45 degrees
C was enhanced to 140 at 5 degrees C in a typical example.