ULTRASTRUCTURAL-CHANGES IN NITRIC-OXIDE SYNTHASE IMMUNOREACTIVITY IN THE BRAIN OF RATS SUBJECTED TO PERINATAL ASPHYXIA - NEUROPROTECTIVE EFFECTS OF COLD TREATMENT

Striatal and cortical neurons containing nitric oxide synthase (NOS) w ere studied in adult rats subjected to different periods of perinatal asphyxia (PA) using immunohistochemistry at both light microscopy (LM) and electron microscopy (EM). Another group was subjected to PA + hyp othermia to study its neuroprotective effect. Quantitative image analy sis was performed on the striatum and neocortex in order to count the number of immunoreactive neurons and to compare the pattern of stainin g between the different groups. Six-month-old rats that suffered subse vere and severe PA demonstrated, at LM, cytomegaly of the striatal and neocortical neurons containing NOS. Control and hypothermic neurons w ere more weakly immunostained than PA neurons. Subsevere and severe as phyctic rats showed an important neuronal loss that was reduced by hyp othermic treatment. The PA group disclosed, at EM, dense electronic bo dies distributed in terminals surrounding synaptic vesicles and in den drites. Non-NOS-containing neurons showed signs of degeneration, such as dark cytoplasm and shrunken nuclei. Surrounding the blood vessels, we observed a clear edema. The immunolabeling in hypothermic rats rese mbled that observed in controls. These data suggest that subsevere and severe PA induces chronic changes in the neuronal content of NOS in t he striatum and neocortex. Degeneration observed in neurons surroundin g cytomegalic NOS-containing cells may be due to the excess of NO in t heir environment. Moreover, the chronic alterations produced by PA see m to be prevented by hypothermia. (C) 1997 Elsevier Science B.V.