USE OF GREEN FLUORESCENT PROTEIN TO DETECT EXPRESSION OF NIF GENES OFAZOARCUS SP. BH72, A GRASS-ASSOCIATED DIAZOTROPH, ON RICE ROOTS

Authors
EGENER T HUREK T REINHOLDHUREK B
Citation
T. Egener et al., USE OF GREEN FLUORESCENT PROTEIN TO DETECT EXPRESSION OF NIF GENES OFAZOARCUS SP. BH72, A GRASS-ASSOCIATED DIAZOTROPH, ON RICE ROOTS, Molecular plant-microbe interactions, 11(1), 1998, pp. 71-75
Citations number
31
Categorie Soggetti
Plant Sciences","Biothechnology & Applied Migrobiology",Biology
Journal title
Molecular plant-microbe interactions → ACNP
ISSN journal
08940282
Volume
11
Issue
1
Year of publication
1998
Pages
71 - 75
Database
ISI
SICI code
0894-0282(1998)11:1<71:UOGFPT>2.0.ZU;2-N
Abstract
A gfp (green fluorescent protein) cassette for transcriptional fusions has been developed to study gene expression in Azoarcus sp. BH72 in a ssociation with plant roots. The bacteria expressed nitrogenase genes (nifHDK) in the rhizosphere, on root tips, and in epidermal cells of r ice seedlings. Green fluorescent protein fusions also visualized promo ter activity of single cells in soil.