Two new Streptomyces phages, oBP1 and oBP2, were isolated from tropica
l soil samples. These phages presented a large host range and develope
d both lyric and lysogenic responses in different Streptomyces species
tested. Variations in the incubation temperature showed to be importa
nt in the development of the replication cycle. Increasing incubation
temperature from 30 degrees C to 42 degrees C induced the lytic respon
se of oBP2 and lysogenic of oBP1 in the host strain Streptomyces sp. W
L6. oBP1 and oBP2 have icosahedral heads with long tails and were char
acterized in relation to morphology, G + C content, genome size and ad
sorption curve.