A. Sakurada et al., INFREQUENT GENETIC ALTERATIONS OF THE PTEN MMAC1 GENE IN JAPANESE PATIENTS WITH PRIMARY CANCERS OF THE BREAST, LUNG, PANCREAS, KIDNEY, AND OVARY/, Japanese journal of cancer research, 88(11), 1997, pp. 1025-1028
In the present study, we searched for genetic alterations of the entir
e coding region of PTEN/MMAC1, a recently isolated candidate tumor sup
pressor gene, in 178 specimens from Japanese patients with various mal
ignant tumors by the polymerase chain reaction-single strand conformat
ion polymorphism method. The samples consisted of 11 glioblastoma mult
iformes (GBMs), 14 astrocytomas, 47 breast cancers, 25 non-small cell
lung cancers, 9 small cell lung cancers, 8 pancreatic cancers, 24 rena
l cell carcinomas, 20 ovarian cancers, and 20 metastatic lung tumors f
rom various organs. Only one somatic frameshift mutation at codon 319
was observed in one (9%) of eleven GBMs. Our results suggest that muta
tion of the PTEN/MMAC1 gene does not play a major role in carcinogenes
is, at least in the tumor types from Japanese patients analyzed in thi
s study.