Mmp. Delcastillo et al., SOME TECHNICAL PRECISIONS TO A METHOD FOR IN-VIVO INTESTINAL-ABSORPTION STUDIES, Journal of physiology and biochemistry, 53(3), 1997, pp. 281-287
Some modifications to the method of PONZ et nl, for in vivo intestinal
absorption studies using an in situ perfused segment of small intesti
ne, under anesthesia, are described. They improve its accuracy and app
licability, especially when slowly absorbed substrates are used or whe
n volume flux measurements are desired. Calculations for the absorbed
substrate and net fluid volume change determinations are reported. Sev
eral aspects of the use of the method under a single pass or a recircu
lation perfusion system are discussed.