THE INFLUENCE OF KINETIN ON THE PROTEIN-SYNTHESIS PATTERN AT SOME STAGES OF THE CELL-CYCLE OF REGENERATIVE SYNCHRONIZED CELL-SUSPENSION CULTURES OF DAUCUS-CAROTA L

Carrot cells (Daucus carota L.) cultured in an IAA and m-inositol cont aining medium were able to produce adventitious roots and somatic embr yos. These differentiational processes could be suppressed by a kineti n supplement, which also increases cell proliferation. In synchronized carrot cell suspensions (using a Fluordesoxyuridin/Thymidine system) in the plus kinetin treatment soon after thymidine application the cel ls passed into the S-phase whereas in the kinetin free nutrient medium the entrance into the S-phase was delayed for about 1 hour during whi ch the cells remained in the G1-phase. During this lag period some 140 proteins are synthesized in a hierachical sequence (C-14-leucine as a tracer), which were missing in the plus kinetin cultures. It remains to be seen to which extent these additional proteins synthesized durin g the prolonged G1-phase contribute to the ability of the cells of thi s treatment to enter the S-phase later and/or the ability of these cul tures to differentiate adventitious roots or somatic embryos.