THE INFLUENCE OF KINETIN ON THE PROTEIN-SYNTHESIS PATTERN AT SOME STAGES OF THE CELL-CYCLE OF REGENERATIVE SYNCHRONIZED CELL-SUSPENSION CULTURES OF DAUCUS-CAROTA L
C. Froese et Kh. Neumann, THE INFLUENCE OF KINETIN ON THE PROTEIN-SYNTHESIS PATTERN AT SOME STAGES OF THE CELL-CYCLE OF REGENERATIVE SYNCHRONIZED CELL-SUSPENSION CULTURES OF DAUCUS-CAROTA L, Journal of Applied Botany-Angewandte Botanik, 71(3-4), 1997, pp. 111-115
Carrot cells (Daucus carota L.) cultured in an IAA and m-inositol cont
aining medium were able to produce adventitious roots and somatic embr
yos. These differentiational processes could be suppressed by a kineti
n supplement, which also increases cell proliferation. In synchronized
carrot cell suspensions (using a Fluordesoxyuridin/Thymidine system)
in the plus kinetin treatment soon after thymidine application the cel
ls passed into the S-phase whereas in the kinetin free nutrient medium
the entrance into the S-phase was delayed for about 1 hour during whi
ch the cells remained in the G1-phase. During this lag period some 140
proteins are synthesized in a hierachical sequence (C-14-leucine as a
tracer), which were missing in the plus kinetin cultures. It remains
to be seen to which extent these additional proteins synthesized durin
g the prolonged G1-phase contribute to the ability of the cells of thi
s treatment to enter the S-phase later and/or the ability of these cul
tures to differentiate adventitious roots or somatic embryos.