In a feeding experiment with 48 male broiler chickens was the effect o
f dietary ethoxyquin and dl-alpha-tocopherol acetate on vitamin E stat
us of chickens investigated. The experimental diets were offered the c
hickens from the first day of age until the end of the experiment at 4
2 days. Chickens were fed on diets containing 0, 75, or 150 mg ethoxyq
uin/kg feed, with (30 mg/kg feed) or without added dl-alpha-tocopherol
acetate. The concentration of alpha-tocopherol in plasma of chickens
on dl-alpha-tocopherol acetate supplemented diets were significantly h
igher than those of chickens fed on the nonsupplemented diets. Supplem
entation with alpha-tocopherol acetate reduced the haemolysis in vitro
. Ethoxyquin seemed to have an increasing effect on the concentration
of cu-tocopherol in plasma, but not in the liver. Addition of ethoxyqu
in to diets without dl-tocopherol acetate reduced the concentration of
gamma-tocopherol in plasma. Significant interactions between the addi
tion of ethoxyquin and the addition of dl-alpha-tocopherol acetate to
the feed were observed on the haemolysis in vitro and the activity of
glutathione peroxidase in plasma. The results indicate that incorporat
ion of ethoxyquin in the diets of chickens exert an improving effect o
n the vitamin E status of the organism.