COMPARATIVE HPLC ANALYSIS OF SEED ALBUMINS FROM VICIA-FABA AND V-KALAKHENSIS (FABACEAE)

Previously reported electrophoretic seed albumin data have shown an un expected association of Vicia faba with V. kalakhensis. In the present work, seed albumins of V. faba (subsp. paucijuga and subsp. faba) wer e compared with those of V, kalakhensis using ion-exchange (IE) and re versed-phase (RP) high-performance liquid chromatography (HPLC). Two s ubspecies of V. faba displayed similar seed albumin profiles. On the o ther hand, seed albumin profiles of V. faba and V. kalakhensis showed no major protein peak in common either in IE-HPLC or RP-HPLC chromatog rams. The reported differences in seed albumin composition of V. faba and V. kalakhensis are consistent with other taxonomical data showing V. faba to be genetically distant from the wild relatives.