Sl. Alvarezcrespo et al., AMPEROMETRIC GLUTAMATE BIOSENSOR BASED ON POLY(O-PHENYLENEDIAMINE) FILM ELECTROGENERATED ONTO MODIFIED CARBON-PASTE ELECTRODES, Biosensors & bioelectronics, 12(8), 1997, pp. 739-747
A glutamate biosensor was developed by electropolymerizing o-phenylene
diamine on a dehydrogenase-NAD(+) modified carbon paste electrode. The
amperometric response to glutamate was obtained by means of the elect
rocatalytic oxidation of the enzymatically produced NADH, at an applie
d potential close to OV (Ag/AgCl). After optimizing carbon paste compo
sition, polymer thickness and operating variables, a linear amperometr
ic response to glutamate was obtained within the concentration range 5
.0 x 10(-6)-7.8 x 10(-5) M with a detection limit of 3.8 x 10(-6) M. T
he biosensor was applied to the determination of glutamate in chicken
bouillon cubes. Good accuracy was found with respect to a reference en
zymatic spectrophotometric method. (C) 1997 Elsevier Science Limited.