SULFATED LEWIS-X DETERMINANTS AS A MAJOR STRUCTURAL MOTIF IN GLYCANS FROM LS174T-HM7 HUMAN COLON-CARCINOMA MUCIN

This article describes oligosaccharide structures of mucin isolated fr om nude mouse xenograft tumors produced by LS174T-HM7 cells, a subline of the human colon carcinoma LS174T with higher metastatic tendency a nd higher mucin production. A striking feature of the oligosaccharides of the LS174T-HM7 xenograft tumor mucin was a predominance of sulfate d Lewis X determinants: HSO3-Gal beta 1-4(Fuc alpha 1-3)GlcNAc. In add ition to one previously known saccharide with one sulfated Lewis X det erminant, the HM7 xenograft tumor mucin contained multiple novel struc tures containing one, two, or three sulfated Lewis X determinants. Thi s determinant, known to act as a selectin ligand, has been found previ ously in minor saccharide components of human milk as well as mucins, but never before as a predominant structure in one mucin source.