A SIMPLE METHOD FOR DETECTION OF HUMAN FACTOR-IX INHIBITOR USING ELISA

Citation
O. Takamiya et S. Kinoshita, A SIMPLE METHOD FOR DETECTION OF HUMAN FACTOR-IX INHIBITOR USING ELISA, Scandinavian journal of clinical & laboratory investigation, 57(8), 1997, pp. 683-688
Citations number
10
ISSN journal
00365513
Volume
57
Issue
8
Year of publication
1997
Pages
683 - 688
Database
ISI
SICI code
0036-5513(1997)57:8<683:ASMFDO>2.0.ZU;2-L
Abstract
We developed a simple ELISA for FIX inhibitors (FIXinh). The relations hip between absorbance and various dilutions of plasma containing know n FIXinh (28 Bethesda units/ml) was linear, ranging from 200 to 25 600 -fold dilution on semi-log paper. Converted into the value of Bethesda Inhibitor Assay, ELISA can detect as low as 0.219 Bethesda units/ml. Absorbance for 30 normal individuals was 0.213+/-0.008, which correspo nds to <0.44 Bethesda units/ml. With ELISA, only the value for hemophi lia B with FIXinh, which was revealed as positive by Bethesda Inhibito r Assay, was significantly higher than that of normal controls, hemoph ilia B without FIXinh, hemophilia A with FVIIIinh and systematic lupus erythematosus (SLE) with lupus anticoagulant (LA). The value in the p atient with SLE, who had a high level of FVIIIinh (1000 Bethesda units /ml), was 3 Bethesda units/ml of FIXinh with Bethesda Inhibitor Assay, which was not significantly higher than that of normal subjects with ELISA. FIXinh in hemophilia B patients by ELISA for FIXinh was not aff ected by the presence of other anticoagulants.