CALCIUM-EVOKED INSULIN RELEASE FROM INSULINOMA CELLS IS MEDIATED VIA CALCIUM-SENSING RECEPTOR

Background. lye have previously reported that high extracellar calcium ([Ca2+]o) levels elicited rapid increases in the cytosolic free calci um ([Ca2+]i) and insullin release from human insulinoma cells. In this study further investigated the mechanism for stimulus-secretion coupl ing of insulinoma cells exposed to high levels of [Cn(2+)]o. Methods. Insulinoma tissues were surgically obtained for primary culture. The c hanges of [Ca2+]i level in response to various agents were monitored b y fluoromety. Total RNA was extracted from tissues and subjected to re verse transcription-polymerase chain reaction (RT-PCR) with calcium-se nsing-receptor (CaR)-specific primers. PCR products were subcloned and sequenced. Results. When [Ca2+]o level was elevated [Ca2+]i in insuli noma cells was immediately increased. Application of neomycin abolishe d the increase In [Ca2+]i level, although extracellular nifedipine and lanthanum chloride did not affect it. The depletion of intracellular calcium stoves with thapsigargin or carbachol eliminated the increase in [Ca2+]i level. RT-PCR analysis identified the 682 bp product, of wh ich the sequence was identical to the corresponding regions of human p arathyroid CaR. Conclusions. Intracellular Ca2+ release might be impor tant in insulin release from insulinoma cells after exposing to high l evel of [Ca2+]o. CaR could be involved in this mechanism.