SCREENING OF ANTICANCER DRUGS BY ACRIDINE ORANGE-CELLULAR DNA FLUORESCENCE INHIBITION

A new method for preliminary screening of anticancer drugs by Hadamard transform microscopic fluorescence image analysis was proposed. Rat l iver 2c hepatocyte was used as the standard cell and acridine orange ( AO) was employed to trace the interaction of drug and cellular DNA. Th e results for five anticancer drugs, vincristine, cyclophosphamide, mu stine, cis-platin and mitomycin-C are in accordance with the mechanism of the drugs binding to cellular DNA and this indicates that this met hod is suitable far screening cell cycle nonspecific anticancer drugs.