Pg. Schneider et Grd. Arnaiz, AREA-DEPENDENT CNS MEMBRANE RESPONSE OF MUSCARINIC RECEPTOR TO CONVULSANT 3-MERCAPTOPROPIONIC ACID, Molecular and chemical neuropathology, 32(1-3), 1997, pp. 213-221
It has already been shown that the administration of convulsant 3-merc
aptopropionic acid at 150 mg/kg enhances binding affinity of muscarini
c antagonist [H-3]quinuclidinyl benzilate ([H-3]QNB) to certain rat CN
S membranes without affecting site number. Herein we employed a 100 mg
/kg dose and tested [3H]QNB binding to cerebellar, hippocampal, and st
riatal membranes obtained from rats killed at preseizure, seizure, and
postseizure stages. In cerebellum, binding increased 24, 65, and 19%
at preseizure, seizure, and postseizure stages, respectively; in hippo
campus, values were 12 and 20% higher at pre-and seizure stages, but f
ailed to differ from controls at postseizure; in striatum, increases o
f 10 and 18% were recorded at seizure and postseizure, with no changes
at preseizure. Neither a subconvulsant dose (20 mg/kg) nor in vitro d
rug addition had any effect on binding. Results indicate a differentia
l response to the convulsant, with reversible changes in cerebellum an
d hippocampus, and a delayed response in striatum, supporting the conc
ept of area-dependent neuronal plasticity.