The karyotypes of the Drosophila robusta species-group, especially its
species-subgroup okadai, were studied by using brain cell-Giemsa stai
ning method. Drosophila gani possessed a diploid number of 12 chromoso
mes, comprising 4 pairs of metacentric and 2 pairs of acrocentric chro
mosomes, and both D. okadai and D. neokadai did 2n = 12, with 3 pairs
of metacentrics, 2 pairs of acrocentrics and 1 pair of microchromosome
s. The okadai subgroup characteristically retained a middle-sized acro
centric X chromosome, which implies that this subgroup may occupy an a
ncestral position for the robusta group.