NOTI LINKING JUMPING CLONES OF HUMAN-CHROMOSOME-3 - MAPPING OF THE TFRC, RAB7 AND HAUSP GENES TO REGIONS REARRANGED IN LEUKEMIA AND DELETEDIN SOLID TUMORS/

By applying the 'recognition mask' strategy to 300 mammalian sequences containing NotI sites we demonstrated that 5' ends of genes are highl y enriched in NotI sites, A NotI linking clone NL2-252 (D3S1678) conta ining transferrin receptor (TFRC) gene was used as an initial point fo r chromosomal jumping, One of the jumping clones, J21-045 traverses 21 0 kbp and links NL2-252 to NL26 (D3S1632), a NotI linking clone contai ning highly polymorphic sequences, The TFRC gene was mapped to 3q29, c lose to the telomeric marker D3S2344, by linkage analysis, a panel of hybrid cell lines, GeneBridge 4 panel and FISH, Clone NLM-007 (D3S4302 ) was found to contain ras-homologous gene RAB7, By FISH and a panel o f hybrid cell lines this gene was mapped to 3q21, This region is of pa rticular interest due to frequent rearrangements in different types of leukemia, Clone L2-081 (D3S4283) containing new member of ubiquitin-s pecific proteases (HAUSP gene) was localized in 3p21 inspiring further investigation of involvement of this gene in development of lung and renal carcinomas. (C) 1997 Federation of European Biochemical Societie s.