CYTOGENETIC ANALYSIS USING SIMULTANEOUS AND SEQUENTIAL FLUORESCENCE IN-SITU HYBRIDIZATION

Authors
ESCUDERO T FUSTER C COLL MD EGOZCUE J
Citation
T. Escudero et al., CYTOGENETIC ANALYSIS USING SIMULTANEOUS AND SEQUENTIAL FLUORESCENCE IN-SITU HYBRIDIZATION, Cancer genetics and cytogenetics, 100(2), 1998, pp. 111-113
Citations number
5
Categorie Soggetti
Oncology,"Genetics & Heredity
Journal title
Cancer genetics and cytogenetics → ACNP
ISSN journal
01654608
Volume
100
Issue
2
Year of publication
1998
Pages
111 - 113
Database
ISI
SICI code
0165-4608(1998)100:2<111:CAUSAS>2.0.ZU;2-P
Abstract
A sequential fluorescence in situ hybridization (FISH) technique is de scribed. This method allows the detection of up to eighteen chromosome pairs in consecutive hybridizations (8 steps) on the same metaphase u sing centromeric, whole chromosome painting, and single copy DNA probe s with different fluorochromes, The technique may be used with diagnos tic purposes in cases with poor cytogenetic material. (C) Elsevier Sci ence Inc., 1998.