CALIBRATION OF ION-EXCHANGE HPLC MEASUREMENTS OF GLYCOHEMOGLOBIN - EFFECT ON INTERASSAY PRECISION

Authors
Citation
E. Gerlo et F. Gorus, CALIBRATION OF ION-EXCHANGE HPLC MEASUREMENTS OF GLYCOHEMOGLOBIN - EFFECT ON INTERASSAY PRECISION, Clinical chemistry, 43(12), 1997, pp. 2353-2357
Citations number
19
Categorie Soggetti
Medical Laboratory Technology
Journal title
ISSN journal
00099147
Volume
43
Issue
12
Year of publication
1997
Pages
2353 - 2357
Database
ISI
SICI code
0009-9147(1997)43:12<2353:COIHMO>2.0.ZU;2-5
Abstract
To investigate the effect of calibration with lyophilized calibrators on the interassay precision of glycohemoglobin (glyHb) measurements, w e used an ion-exchange HPLC system equipped with a Pharmacia Mono S(R) HR 5/5 column. Calibration of analytical runs substantially increased interassay variation (CV), from 1.7% to 4.4% and from 0.9% to 3.2% fo r control samples with low (6.5%) and high (14%) glyHb percentages, re spectively. Standardization of glyHb results, though essential for int erlaboratory comparisons, should not be done at the expense of assay p recision, as may occur with thoughtless use of lyophilized calibrators . We therefore recommend the use of carefully determined conversion fa ctors for standardization of glyHb results obtained with ion-exchange HPLC systems that are capable of excellent long-term interassay precis ion.