F. Frantzen et al., GLYCOHEMOGLOBIN FILTER ASSAY FOR DOCTORS OFFICES BASED ON BORONIC ACID AFFINITY PRINCIPLE, Clinical chemistry, 43(12), 1997, pp. 2390-2396
We present a new filter assay for the determination of glycohemoglobin
as a unique application of the boronic acid affinity principle. With
the use of a water-soluble blue-colored boronic acid derivative and a
specific precipitation method for hemoglobin, total hemoglobin includi
ng bound boronic acid is precipitated and collected on a filter strip
before quantification. Hemoglobin and boronic acid are quantified by a
dual-wavelength reflectometric measurement, and the result is reporte
d directly as percent glycohemoglobin. The test is simple, quick, and
designed as a doctors' office test for the monitoring and management o
f diabetes. The imprecision of the assay is <4% over the range 3-18% H
b A(1c), and the method is linear up to at least 20% Hb A(1c). Compari
sons with four well-established glycohemoglobin methods yielded correl
ation coefficients ranging from 0.94 to 0.99, with slopes from 0.94 to
1.01.