CLEAVAGE OF TRANSCRIPTIONAL ACTIVATOR OCT-1 BY POLIOVIRUS ENCODED PROTEASE 3C(PRO)

In HeLa cells, RNA polymerase II mediated transcription is severely in hibited by poliovirus infection. Both basal and activated transcriptio n are affected to bring about a complete shutoff of host cell transcri ption. We demonstrate here that the octamer binding transcription fact or, Oct-1, is cleaved in HeLa cells infected with poliovirus. Incubati on of Oct-1 with the purified, recombinant 3C(pro) results in the gene ration of the cleaved Oct-1 product seen in virus infected cells. Poli ovirus infection leads to the formation of altered Oct-1-DNA complexes that can also be generated by incubation of Oct-1 with purified 3C(pr o). We also show that Oct-1 cleaved by 3C(pro) loses its ability to in hibit transcriptional activation by the SV40 B enhancer. These results suggest that cleavage of Oct-1 in poliovirus infected cells leads to the loss of its activity. (C) 1997 Academic Press.