Ve. Tikhonov et al., AFFINITY PURIFICATION OF MAJOR CHITINASES PRODUCED BY STREPTOMYCES-KURSSANOVII, Enzyme and microbial technology, 22(2), 1998, pp. 82-85
A highly O-cross-linked chitin-based sorbent resistant toward digestio
n by the extracellular chitinolytic complex of Streptomyces kurssanovi
i was prepared by means of cross-linking of N-acetylchitosan with 2,3-
epoxypropyl chloride. Not less than four stages of cross-linking were
required for the matrix to be fully resistant toward the action of chi
tinolytic enzymes. The sorbent was shown to be specific for two major
endochitinases which were isolated in homogeneous forms quantitatively
. (C) 1998 Elsevier Science Inc.