ESTIMATION OF PARAMETERS FOR CELL-SURFACE INTERACTIONS - MAXIMUM BINDING FORCE AND DETACHMENT CONSTANT

A convenient model is presented which can be used to quantify the rela tionship between applied shear and attached cell fraction in cell/surf ace interaction studies The model uses two parameters (the shear stres s required to detach the total attached cell population and a detachme nt constant) based on the estimated strength of the cell/bead interact ion force. Use of these parameters allows results obtained on differen t systems to be compared. The model has been applied to data from thre e systems. 1) The effects of shear on the interaction between anti-goa t IgG-coated beads and surface immobilized goat IgG;(1) 2) The effect of applying fluid shear stress to a stable fraction of attached 3T3 fi broblast cells on glass;(2) and 3) The interaction of suspended yeast cells with surface-immobilized concanavalin A which is reported here. In the yeast system the model provided a convenient aid for quantifyin g the effect of competing glucose on the interaction strength where it was found that the detachment constant for yeast interaction with sur face-bound conA increases with the glucose concentration while the max imum shear stress and the binding force between the yeast cells and co nA decreases. (C) 1998 Elsevier Science Inc.