ISOZYMIC AND METRIC VARIATION IN THE LUTZOMYIA-LONGIPALPIS COMPLEX

Male Lutzomyia longipalpis of two types from Bolivia were compared usi ng isozyme electrophoresis and wing morphometry. One sample (ex Chiflo nkaka Cave, all. 2800 m at Toro Toro, Charcas Province, Potosi Departm ent) was 'two-spot' phenotype males (i.e. tergites m and IV with paire d pale patches of pheromone glands), whereas two other locality sample s (Apa Apa and Imanaco, Sud Yungas Province, La Pat Department) were o ne-spot male phenotype (only tergite IV with paired pale patches). Mul tilocus enzyme electrophoresis (using ACON, aGPD, GPI, IDH, MDH, ME, 6 PGD, PGM, LAP and PEPB) found no difference between samples from adjac ent hen houses at Apa Apa. Nei's standard genetic distance between one -spot samples from Apa Apa and Imanaco (5 km apart, 1500m ah.) was 0.0 01-0.002, whereas the two-spot males from Toro Toro (800km away) showe d a genetic distance of 0.081 from the one-spot males (Apa Apa and Ima naco). This genetic distance is commensurate with speciation, but may simply be intraspecific differentiation due to 'isolation by distance' . For comparative wing morphometry, we included additional material of one-spot males from Bolivia (Guyabal, Sud Yungas, La Pat), Brazil, Co lombia and Nicaragua. These three other country samples were assumed t o be different sibling species in the complex Lutzomyia longipalpis (L anzaro et al., 1993). Statistics were based on univariate and multivar iate analysis. The comparison between size-in and size-free canonical variate analysis (CVA) indicated that the wing morphometric divergence between one-spot and two-spot Bolivian phenotypes was not size depend ent and could have taxonomic significance.