R. Peeters et al., EVIDENCE FOR THE PRESENCE OF IMMUNOREACTIVE INHIBIN IN EXTRAGONADAL TISSUES OF OVARIECTOMIZED EWES, Animal reproduction science, 48(2-4), 1997, pp. 257-268
Six ewes were ovariectomized to determine the immediate and long-term
effects of removal of ovaries on the immunoreactive concentrations of
FSH, LH and inhibin. Three months after ovariectomy, ewes were slaught
ered and tissue samples of brain, pituitary, spleen, liver, perirenal
fat, lung, kidney, adrenals and uterus were collected to determine the
immunoreactive inhibin content, Both gonadotrophins, FSH and LH, incr
eased significantly after ovariectomy. The increase of FSH, however, w
as more pronounced and remarkably faster than the changes of LH after
ovariectomy. Immunoreactive concentrations of inhibin decreased sharpl
y as early as 15 min after ovariectomy and subsequently decreased more
gradually until 2 weeks after surgery, From this moment on, the level
stabilized at 56% of the initial value. In control ewes, a considerab
le amount of immunoreactive inhibin is found in tissue samples of ovar
y, lung, kidney, pituitary and spleen. After ovariectomy, the level of
immunoreactive inhibin decreased in spleen and lung samples while an
important increase of immunoreactive inhibin is found in adrenals and
pituitary. These results demonstrate a differential regulation of LH a
nd FSH after ovariectomy and support an involvement of inhibin only in
the immediate changes of FSH after ovariectomy in sheep. They further
suggest that the adrenals and the pituitary may be extragonadal sourc
es of inhibin. To explore the eventual contribution of the adrenals to
circulating inhibin, dexamethasone (1.4 mg/ewe) and ACTH (200 IU/ewe)
were in following experiment injected intravenously in control and ov
ariectomized ewes. The lack of any effect of dexamethasone: or ACTH on
the plasma concentration of immunoreactive: inhibin indicate that adr
enal inhibin probably does not contribute to circulating inhibin. (C)
1997 Elsevier Science B.V.