MILLIMETER WAVES INHIBIT THE SYNERGISTIC REACTION OF CALCIUM IONOPHORE A23187 AND PHORBOL ESTER IN NEUTROPHIL RESPIRATORY BURSTS

The effect of extremely high frequency electromagnetic field (mm-waves ) on respiratory burst of neutrophils was studied. The peritoneal evok ed neutrophils of the mice (NMRI line) were used. The production of re active oxygen species was estimated by luminol-dependent chemiluminesc ence technique. Cells were irradiated by the mm-waves of 41,95 GHz in the far field zone of the channel radiator during 20 min. Absorbed ene rgy flux density was 150 mu W/cm(2). The irradiation was carried out a t different concentrations of calcium ionophore A23187 and then neutro phils were stimulated by phorbol 12-myristate 13-acetate (PMA) 1 mu M, activator of PKC. At irradiation of neutrophils the synergistic actio n of A23187 and PMA was not changed at low concentrations of ionophore 10 nM-0,5 mu M and was suppressed at high concentrations 0,5-10 mu M. The largest inhibition of about 60% was obtained at the concentration of A23187 20 mu M. The effect of mm-waves was not found under exposur e in Ca2+-free medium for all used A23187 concentrations. We suggest t hat the mm-wave effect;on the production of reactive oxygen species by neutrophils is determinated by the influx of extracellular Ca2+ and P KC.