Am. Talaat et al., IDENTIFICATION OF MYCOBACTERIA INFECTING FISH TO THE SPECIES LEVEL USING POLYMERASE CHAIN-REACTION AND RESTRICTION ENZYME ANALYSIS, Veterinary microbiology, 58(2-4), 1997, pp. 229-237
An assay is described utilizing PCR technology for a rapid diagnostic
test to identify fish infection with Mycobacterium marinum, M. fortuit
um and hi. chelonae. A 924 bp DNA fragment from a highly conserved are
a of the mycobacterial 16S rRNA gene was amplified using mycobacteria
genus-specific primers and digested with restriction enzymes (BanI and
ApaI). This examination yielded unique restriction patterns for each
mycobacterial specie enabling identification of mycobacteria infecting
fish to the species level. The protocol can be applied to purified DN
A, a simple colony preparation or infected fish tissue. This protocol
can be completed in 1-2 days. (C) 1997 Elsevier Science B.V.